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Vip3 ELISA kit
Vip3A ELISA test kit is intended to be used for the qualitative detection of Vip3A protein in individual corn leaf or seed samples and bulk grain corn at 0.2% (1 Vip3A Positive seed in 500 conventional seeds).
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Different quantities are also available in 10/50 plate formats.
For more information or to request a quote, contact us now.
An antibody specific to the Vip3A protein molecule is immobilized on the microwell plates and the second antibody
specific for Vip3A molecule is conjugated with horse radish peroxidase (HRP). When the sample extracts are
added to the microtiter wells, the Vip3A from sample binds to the antibody immobilized in the well. This binding
is subsequently detected by addition of enzyme-labeled antibody. After a washing step, the substrate is added. The
enzymatic reaction and development of color are proportional to the amount of Vip3A present in the sample. The
reaction is terminated by the addition of stop solution. Absorbance is then measured on a plate reader.
| SKU | AID 059 |
|---|---|
| No. of tests | 96 |
| Specificity | Qualitative detection of Vip3A protein in individual corn leaf or seed samples and bulk grain corn at 0.2% (1 Vip3A Positive seed in 500 conventional seeds) |
| Relevant species | Corn leaf, seed and bulk grain |
| Cross-reactivities | The Vip3A ELISA kit does not recognize CP4EPSPS (RoundUp Ready), Cry1F, Cry2A, Cry1Ac/Ab or PAT/pat (LibertyLink***) |
| Assay time | 60 min |
| Storage | 2-8 °C |