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Cry3Bb ELISA Kit
Cry3Bb ELISA test kit is intended to be used for the qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Rootworm RW event 863. The test is used to detect the Cry3Bb protein in individual Maize seed or leaf. It can also be used to detect event 863 in bulk grain at 0.2% (1 positive in 500 negative seeds)
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Different quantities are also available in 10/50 plate formats.
For more information or to request a quote, contact us now.
An antibody specific to the Cry3Bb protein molecule is immobilized on the microwell plates and the second antibody
specific for Cry3Bb protein is conjugated with horse radish peroxidase (HRP). When the sample extracts are
added to the microtiter wells, the Cry3Bb from the sample binds to the antibody immobilized in the well. This binding
is subsequently detected by addition of enzyme-labeled antibody. After a washing step, the substrate is added. The
enzymatic reaction and development of color are proportional to the amount of Cry3Bb present in the sample.
The reaction is terminated by addition of stop solution. Absorbance is then measured on a plate reader.
| SKU | AID 053 |
|---|---|
| No. of tests | 96 |
| Specificity | Qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Rootworm RW event 863 |
| Relevant species | Corn leaf, seed and bulk grain |
| Cross-reactivities | The Cry3Bb ELISA kit does not cross-react with CP4EPSPS (Roundup Ready**), Cry1F, Cry1Ac, eCry3.1Ab (AgriSure Duracade), mCry3A, Cry1Ab, Cry2A, Vip3A, Cry34Ab1, Cry35Ab1 or PAT (LibertyLink***) |
| Assay time | 60 min for leaf/seed & 90 min for bulk grain |
| Storage | 2-8 °C |