I'screen SULFA, ELISA, 96 det.

ELISA test kit for the detection of sulphonamides.

ELISA test kit for the detection of sulphonamides.

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SKU	HU0050003
Assay principle	Microplate competitive enzyme immunoassay.
Format	Microtiter plate with 96 wells
Assay time	75 min
Analysable samples	Muscle, Egg, Milk, Honey, Feed
Detection limit (LOD)	Muscle, Egg (Method I), Honey (Method III, high sensitivity) - 0.5 ppb Milk (Method I) - 0.25 ppb Muscle, Egg, Milk, Honey (Method II, solvent-free) - 5 ppb Feed - 66.6 ppb
Sample preparation	Muscle, Egg, Milk (Method I, High Sensitivity) - Homogenization, (Skimming for milk), Solvent Extraction, Centrifugation, Evaporation Muscle, Egg, Honey (Method II, Solvent Free) - Homogenization, Buffer Extraction (Acid Hydrolysis for Honey), Centrifugation, Filtration Milk (Method II) - Centrifugation, Dilution Honey (Method III) - Acidic Hydrolysis, Purification on SPE Column, Elution, Evaporation, Resuspension Feed - Grinding, Solvent Extraction, Dilution
Specificity	Sulfamerazine >100% Sulfamonomethoxine >100% Sulfadiazine 100% Sulfachloropyridazine 100% Sulfamethoxypyridazine (Sulfapyridazine) >100% Sulfamethoxydiazine (Sulfamethoxine, Sulfameter) >100% Sulfadimethoxine >100% Sulfaquinoxaline >100% Sulfathiazole >100% Sulfamethizole >100% Sulfamethazine 100% Sulfamethoxazole 90% Sulfisoxazole 50% Sulfapyridine 36% Sulfadoxine 24% Sulfacetamide 2.5% Sulfaphenazole <2% Sulfabenzamide <2% Sulfaguanidine <0.1% Sulfanilamide <0.1%