B ZERO® SULFA, ELISA, 48 det.

ELISA test kit for the detection of sulphonamides.

Advantages: B ZERO^® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.

Kit Specifications

More Information
SKU	HU0050006
Assay principle	Microplate competitive enzyme immunoassay.
Format	Microtiter plate with 48 wells
Assay time	75 min
Analysable samples	Muscle, Egg, Milk, Honey, Feed
Detection limit (LOD)	Muscle, Egg (Method I), Honey (Method III, high sensitivity) - 0.5 ppb Milk (Method I) - 0.25 ppb Muscle, Egg, Milk, Honey (Method II, solvent-free) - 5 ppb Feed - 66.6 ppb
Sample preparation	Muscle, Egg, milk (METHOD I, high sensitivity): Homogenization, (skimming for milk), solvent extraction, centrifugation, evaporation. Muscle, egg, honey (METHOD II, solvent free): Homogenization, (acid hydrolysis for honey), buffer extraction, centrifugation, filtration. Milk (Method II) - Centrifugation, Dilution Honey (Method III) - Acidic Hydrolysis, Purification on SPE Column, Elution, Evaporation New line for Feed - Grinding, Solvent Extraction, Dilution
Specificity	Sulfamerazine >100% Sulfamonomethoxine >100% Sulfadiazine >100% Sulfaclozine (Sulfachloropyrazine) > 100% Sulfachloropyridazine >100% Sulfamethoxypyridazine (Sulfapyridazine) >100% Sulfamethoxydiazine (Sulfamethoxine, Sulfameter) >100% Sulfadimethoxine >100% Sulfaquinoxaline >100% Sulfathiazole >100% Sulfamethizole >100% Sulfamethazine 100% Sulfamethoxazole 90% Sulfisoxazole 50% Sulfapyridine 36% Sulfadoxine 24% Sulfacetamide 2.5% Sulfaphenazole <2% Sulfabenzamide <2% Sulfaguanidine <0.1% Sulfanilamide <0.1%

Product Attachments

I'screen SULFA, ELISA, 96 det.

B ZERO® SULFA, ELISA, 48 det.

Test Instruction