Maduramicin, ELISA, 96-test

The Maduramicin ELISA is an immunoassay for the quantitative and sensitive screening of Maduramicin. This test is suitable for the quantitative and/or qualitative screening of Maduramicin in animal feed and contaminated products (please refer to the appropriate technical bulletins for additional extraction/dilution procedures). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Maduramicin by specific antibodies. Maduramicin, when present in a sample, and a Maduramicin-HRP analogue compete for the binding sites of rabbit anti-Maduramicin antibodies in solution. The Maduramicin antibodies are then bound by a second antibody (donkey anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Maduramicin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.