I'screen ZERANOL, ELISA, 96 det.

ELISA test kit for the quantitative detection of corticosteroids

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SKU	HU0050023
Assay principle	Microplate competitive enzyme immunoassay.
Format	Microtiter plate with 96 wells
Assay time	105 min
Analysable samples	Muscle, Urine, Liver
Detection limit (LOD)	Muscle, Liver - 0.025 ppb Urine - 0.25 ppb
Sample preparation	Urine: centrifugation, dilution, enzymatic hydrolysis, purification with C18 columns, evaporation, reconstitution Muscle: homogenization, solvent extraction, centrifugation, evaporation, reconstitution, purification with C18 column Liver: homogenization, enzymatic hydrolysis, solvent extraction, centrifugation, evaporation, reconstitution, centrifugation, purification with C18 column.
Specificity	Zeranol 100% Zearalanone 522% Taleranol (β-zearalanol) 110% α-zearalenol 61% β-zearalenol 6.8% HEX <0.05% DES <0.05% Dienestrol <0.05% 17-β-estradiol <0.05% Progesterone <0.05% Testosterone <0.05% Clenbuterol <0.05% Trenbolone <0.05% Dexamethasone <0.05%