I'screen CAP v2, ELISA, 96 det.

ELISA test kit for the quantitative detection of chloramphenicol.

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SKU	HU0050002
Assay principle	Microplate competitive enzyme immunoassay.
Format	Microtiter plate with 96 wells
Assay time	45 min
Analysable samples	Urine, Water, Serum and Plasma, Tissue, Honey, Seafood, Egg
Detection limit (LOD)	Urine (Method II), Serum, Plasma, Water - 0.1 ppb Urine (Method I) - 10 ppb Honey, Egg - 0.05 ppb Muscle, Seafood - 0.025 ppb
Sample preparation	Urine (Method I) - Centrifugation, Dilution Urine (Method II) - pH adjustment, enzymatic hydrolysis, solvent extraction, evaporation, reconstitution Water: pH adjustment, dilution (optional) Serum & Plasma - Solvent extraction, centrifugation, evaporation, reconstitution Eggs, tissue and seafood - homogenization, solvent extraction, centrifugation, evaporation, reconstitution Honey - Dilution, solvent extraction centrifugation, evaporation, reconstitution
Specificity	Chloramphenicol 100% Chloramphenicol Glucuronide 70% Florophenicol <0.1% Tiamphenicol <0.1%