Benzo(a)Pyrene (B(a)P), ELISA, 96-test

The Benzo(a)Pyrene ELISA is an immunoassay for the quantitative and sensitive detection of Benzo(a)Pyrene in water samples. Sample concentration is not required prior to analysis for LOD of 0.3 ppb. If lower LOD are required a simple concentration step is necessary. If necessary, positive samples can be confirmed by HPLC or other conventional methods. The test is a direct competitive ELISA that allows the detection of Benzo(a)Pyrene. It is based on the recognition of Benzo(a)Pyrene by specific antibodies. Benzo(a)Pyrene, when present in a sample, and a Benzo(a)Pyrene-HRP analogue compete for the binding sites of mouse anti-Benzo(a)Pyrene antibodies in solution. The Benzo(a)Pyrene antibodies are then bound by a second antibody (goat anti-mouse) immobilized in the plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of the Benzo(a)Pyrene present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.